This module aims to provide the student with; Knowledge of the principles of separations systems such as HPLC, GC and Capillary Electrophoresis and their hyphenated techniques. An appreciation of the importance of sample preparation prior to analysis and selection of appropriate extraction method. An understanding of the theory and instrumental operation of spectroscopic methods such as mass spectrometry and UV/Visible spectroscopy and the ability to perform structural identification and purity testing of simple organics using mass spectrometry. The ability to critically analyse data and interpret results.
HPLC:
Instrumentation review; modes of HPLC (reverse phase/chiral separations); method development & applications; standard addition, internal standards, validation, preparative HPLC; practical problems/troubleshooting & maintenance; UV/Visible: instrumentation, chromophores, quantitative analysis.Sample Preparation extraction (liquid-liquid and solid phase), efficiency and selectivity of extraction, supercritical fluid, membrane technology; applications.
Gas Chromatography:
Review of chromatography principles; instrumentation; capillary & packed columns; detectors; method development; quantitative techniques; internal and external calibration; response factors; validation troubleshooting; case studies.
Capillary Electrophoresis:
Capillary Electrophoresis: Instrumentation, principles, theory, EOF, efficiency and resolution, temperature and voltage effects, detectors, modes of CE, applications and case studies
Mass Spectroscopy:
Mass spectrometry: principles of Mass spectrometry; instrument overview; ionisation methods; mass analysers; ion collection systems; structural identification. GC & HPLC-MS: GC-MS and HPLC-MS Overview; interfaces; applications, case studies.
Lecture-based instruction - Traditional Lectures; Small group work, Digital Learning
Individual learning - Small group work, Digital Learning.
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 70 |
| Other Assessment(s) | 30 |