This module aims introduce the student cell cycle regulation. The critical role of signal transduction and protein phosphorylation in cell cycle regulation pathways will be discussed. Pathogenic effects of alterations in cell cycle control will be evaluated. The module also aims to evaluate the principles of gene cloning and the technology involved, including PCR, Sanger and next generation sequencing. The student will be introduced to the application of molecular technologies in the diagnostic laboratory.
- Cell cycle
- Cell signalling systems
- Protein phosphorylation
- Cellular dysregulations and an introduction to oncogenesis
- Methodologies involved in examining cell regulation
- Cell based and in vitro gene cloning methodologies
- Principles and applications of the polymerase chain reaction including end point PCR real time PCR, RT-PCR, ARMS PCR
- Sanger DNA sequencing
- An introduction to next generation sequencing
- Recombinant protein production
This module will be delivered over one semester with a total of 45 contact hours. The module will use a mixture of lecture (20h), practical laboratory work (21h) and tutorials (4h).
|Module Content & Assessment