The module aims to provide the students with: a knowledge of analytical measurement systems, theory of chromatographic methods, HPLC and GC techniques; the ability to carry out simple quantitative and qualitative analysis; the ability to apply chromatographic techniques to analytical laboratory environments; experience of designing computerised measurement systems;
Introduction to HPLC
Instrumental layout / Pumps / Gradients vs. Isocratic / Sample Injectors / Tubing and Connections / Pre- and Analytical Columns / Standard Detectors / Separation Modes (Size Exclusion, Ion Exchange, Speciality, Normal Phase and Reverse Phase) / Polarity / Column Ovens / Solvents / Trouble shooting / Sample Preparation (filtration, liquid-liquid extraction, Solid Phase Extraction);sample preparation; elution; column classification; detectors; trouble-shooting.
Chromatographic methods
Classification / Chromatograms / Chromatographic behaviour of solutes / Efficency and Resultion / Qualitative & Quantitative analysis (internal and external standards);
Introduction to GC
System / Components / Separation sequence / Injection Port / Columns (Packed and Capillary) / Oven (temp. programming) / Standard Detectors / Qualitative & Quantitative analysis (response Factors) / Trouble-shooting.
Computerised Measurement Systems
Signals and Signal Characteristics / Noise / Filters & Amplifiers / Digitisation / Digital Communication Protocols / Light, Temperature, and Pressure Transducers / Digital Image characteristics / malware.
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Other Assessment(s) | 30 |
| Formal Examination | 70 |