Module Overview

DNA Analysis I

This module has been designed to build on core competencies in DNA extraction and amplification achieved in semester 4. The module will cover the techniques of DNA hybridization (including probe design and labelling), and of DNA sequencing both of which underpin genetic analysis. Following on from the fundamentals of PCR covered in semester 4, this module also covers applications of DNA profiling methodologies to microbial, animal and food samples. Theoretical aspects of this module are complemented by laboratory experiments delivered concurrently.

Module Code

DNAA H3002

ECTS Credits


*Curricular information is subject to change

DNA amplification - optimization and adaptations

multiplex PCR, long-range PCR, nested PCR, reverse transcription PCR

Microbial DNA typing

Microbial typing of clinical isolates and industrial samples. Non-PCR based typing(PFGE, RAPD, plasmid profiling, ribotyping)and PCR based typing methods (amplification of known and random sequences).

Electrophoretic techniques in DNA analysis

Agarose gel electrophoresis (AGE), polyacrylamide gel electrophoresis (PAGE), pulse field gel electrophoresis (PFGE), and capillary zone electrophoresis (CZE)

DNA sequencing

Dideoxy Sanger sequencing; principles of automated sequencing; the human genome project

DNA hybridisation techniques

Probe design, labelling and detection methods; factors affecting hybridization reactions; hybridization formats including Dot/Slot/Southern blotting

Module Content & Assessment
Assessment Breakdown %
Formal Examination70
Other Assessment(s)30