This module has been designed to build on core competencies in DNA extraction and amplification achieved in semester 4. The module will cover the techniques of DNA hybridization (including probe design and labelling), and of DNA sequencing both of which underpin genetic analysis. Following on from the fundamentals of PCR covered in semester 4, this module also covers applications of DNA profiling methodologies to microbial, animal and food samples. Theoretical aspects of this module are complemented by laboratory experiments delivered concurrently.
DNA amplification - optimization and adaptations
multiplex PCR, long-range PCR, nested PCR, reverse transcription PCR
Microbial DNA typing
Microbial typing of clinical isolates and industrial samples. Non-PCR based typing(PFGE, RAPD, plasmid profiling, ribotyping)and PCR based typing methods (amplification of known and random sequences).
Electrophoretic techniques in DNA analysis
Agarose gel electrophoresis (AGE), polyacrylamide gel electrophoresis (PAGE), pulse field gel electrophoresis (PFGE), and capillary zone electrophoresis (CZE)
DNA sequencing
Dideoxy Sanger sequencing; principles of automated sequencing; the human genome project
DNA hybridisation techniques
Probe design, labelling and detection methods; factors affecting hybridization reactions; hybridization formats including Dot/Slot/Southern blotting
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 70 |
| Other Assessment(s) | 30 |