This module will be based on practical laboratory experience. It is intended that this practical module will cover laboratory based topics from modules in 'Materials and Trace Analysis', 'Pharmacology' and 'DNA Analysis'. In all practicals, students will be encouraged to develop their ability to integrate the use of various laboratory techniques in problem solving. The concept of team-work, good laboratory practice and the need for both validation and statistical evaluation of results will be emphasized throughout.
Materials and Trace Analysis
Retrieval, identification, sorting, examination and analysis of material and trace evidence in the form of hair, fibre, paint, glass and FAR (Fire Arms Residue) using stereomicroscope, comparison microscopes, polarising microscope, SEM/EDX (Scanning Electron Microscope and Energy Dispersive X-Rays) and GRIM (Glass Refractive Index Measurement).
Pharmacology
Preparation and analysis of samples; Preparation (solid phase or liquid-liquid extraction) and chromatographic / electrophoretic analysis of pharmacologically relevant compounds (and metabolites) from biological samples; Analysis of food and environmental samples for a selection of heavy metals & residues; Use of standard bioassays to establish in vitro toxicity of defined compounds; In vitro assays using liver microsomal fraction and chromatographic analysis to tract the production of Phase I matabolites.
DNA Analysis I
Bacterial DNA isolation; Spectroscopic and Electrophoretic analysis of DNA; Polymerase Chain Reaction (PCR) adaptations e.g. multiplex PCR; Typing of microbial species e.g. PCR RFLP, PCR Ribotyping, RAPD
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Other Assessment(s) | 100 |