This module aims to provide the student with the knowledge and practical skills necessary for the analysis of pharmaceutical compounds by a variety of chromatographic techniques.
Chromatographic Theory
Theory of chromatography, types of chromatographic separations, Van Deemter equation, column efficiency, column performance parameters, industrial based problems.
HPLC
Columns - column packing & construction, normal vs reverse phase, ion pair chromatography, analytical vs. preparative Detectors - photometric, photo diode array (PDA), refractive index, IR, conductivity, fluorescence, electrochemical, hyphenated techniques (HPLC-Mass Spec.)
GC
GC - chromatographic principles, instrumentation, sample derivitisation; columns, gas solid chrom., detectors, quantitative/qualitative analysis (precision, detection limits)
Chromatographic method development & validation
Method development - column selection, use of ion pairing agents, chiral separations, detector selection, separation optimisation, qualitative & quantitative analysis, strategic approach to troubleshooting, practical problems; computer operated systems, application in biopharmaceutical /pharmaceutical analysisMethod validation – linearity, LOD, LOQ, column capacity, inter- & intra-assay validation, stability studies (standards & extracted samples), routine validation
Revision of Calculations
Revision of calculations relevant to the practical course and also introduction to report writing for instrumentation practicals
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Other Assessment(s) | 30 |
| Formal Examination | 70 |