The aim of this module is to provide the student with the fundamentals of the application of spectroscopy and electrochemistry in DNA an Forensics. This module will cover the scope and theory of Ultraviolet/visible spectroscopy, Infrared Spectroscopy, Atomic absorption spectroscopy, NMR Spectroscopy, Mass Spectroscopy and Electrochemical techniques. The module will be delivered with a strong emphasis on the applications of these analytical techniques in DNA and Forensics. Theoretical aspects of this module are underpinned by laboratory experiments from the Laboratory Skills for DNA and Forensics.
UV-Visible Spectroscopy:
Chomophores; Quantitative analysis; Beer-Lambert Law; Single & Double beam instruments; light sources and monochromators; applications.
Infrared Spectroscopy:
Theory; modes of vibration; sample preparation; structural elucidation; applications.
Atomic Spectroscopy:
Techniques; atomization; component parts; interference; sample preparation; quantitative analysis and applications
Potentiometric Techniques:
Reference & indicator electrodes; Nernst equation; pH electrodes; ion selective electrodes; acid-base titrations; quantitation; environmental applications
Mass Spectroscopy:
Theory, Techniques, Molecular Ions, Fragmentation Patterns, Elucidation of Molecular Structures
NMR (1H and 13C)
Theory, Chemical Shifts, Shielding and De-Shielding, Integration, Splitting patterns, Elucidation of Molecular Structures
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Other Assessment(s) | 30 |
| Formal Examination | 70 |