The module aims to provide the student with knowledge of the equipment and technology required for the manufacture of pharmaceutical products
Review of the cell and biomolecules
Prokaryotic and Eukaryotic cells, Transcription and Translation, Recombinant DNA and Proteins. Cell growth kinetics
Bioreactor technology
Reactor design considerations. Control of fermentation process e.g. aeration, pH, agitation and foam control. Media considerations
Isolation and improvement of industrial producer cells
Isolation, screening and selection of producer cells. Appropriate selection of preservation techniques and creation of cell banks. Strain improvement techniques for microbial and mammalian cells. Media considerations
Downstream processes
Crude purification: filtration, TFF, centrifugation. High level purification: chromatography, affinity, ion exchange, size exclusion,hydrophobic interaction. Media considerations
Scale up of bioprocesses
Lab scale. Pilot plant. Production scale. Scale up issues
Case Studies and current trends
Biopharmaceutical products produced from microbes, plant and mammalian cell cultures - growth factors, recombinant antibodies, vaccines,CRISPR
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Other Assessment(s) | 50 |
| Formal Examination | 50 |