This module builds on the skills and knowledge gained by students in DNA analysis I. The objective is to give the student a clear understanding of the principles of DNA based diagnostics and genetic analysis. In addition, emphasis will be placed on new developments which enhance and broaden currently used diagnostic systems.
Nucleic Acid Preparation and Analysis
Extraction of nucleic acids from prokaryotic and eukaryotic samples, quantification and quality assessment of DNA and RNA, Probe and Primer Design.
Advanced DNA amplification based techniques:
Real-time PCR principles and procedures.Applications in human genomic analysis, gene expression studies and microbial epidemiology.
Advanced probing based technologies:
DNA microarray technology - applications in genome analysis, in gene expression studies and in pathogen characterisation.In-situ hybridisation techniques in human genetics and pathogen diagnosis.
DNA Sequencing technologies:
Automated sequencing methods with a focus on Illumina sequencing technologies. MLST analysis. Applications of DNA sequencing to disease diagnosis, forensic genetics and pathogen detection.
DNA analysis in Agri-Food sector
DNA profiling of animals (breeding programs, endangered species investigations).DNA profiling of food samples (species identification and authentication, traceability, GM analysis, microbial contamination)
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 70 |
| Other Assessment(s) | 30 |