This module builds on the skills and knowledge gained by students in DNA analysis I. The objective is to give the student a clear understanding of the principles of DNA based diagnostics and genetic analysis. In addition, emphasis will be placed on new developments which enhance and broaden currently used diagnostic systems.
Advanced DNA amplification based techniques:
Real-time PCR principles and procedures.Applications in human genomic analysis, gene expression studies and microbial epidemiology.
Advanced probing based technologies:
DNA microarray technology - applications in genome analysis, in gene expression studies and in pathogen characterisation.In-situ hybridisation techniques in human genetics and pathogen diagnosis.
DNA Sequencing technologies:
Automated sequencing methods. Second generation sequencing technologies. MLST analysis. Applications of DNA sequencing to disease diagnosis, forensic genetics and pathogen detection
DNA analysis of livestock and food samples
DNA profiling of animals (breeding programs, endangered species investigations).DNA profiling of food samples (species identification and authentication, traceability, GM analysis, microbial contamination)
Bioinformatics:
Databases / Sequence and homology analysis / Restriction site searching / Translation of DNA sequence to protein sequence / Structural analysis of protein sequences / Motif searching
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 70 |
| Other Assessment(s) | 30 |