Module Overview

Applied Biotechnology

This module aims to provide the student with: An understanding of and competency in recombinant DNA technology, A knowledge of the basic concepts of mammalian cell culture; An understanding of the principles of protein isolation and purification, and the necessary skills to isolate and characterise proteins; An understanding of protein diseases and of the roles played by proteins in medicine; Theoretical aspects of this module are complemented by laboratory experiments delivered the Laboratory skills module running concurrently.

Module Code

PHAR H3042

ECTS Credits


*Curricular information is subject to change

DNA Cloning

DNA isolation and analysis; restriction and ligation of DNA, selection of recombinants; recombinant technology

Basic Mammalian Cell Culture

Introduction to cell culture; primary cells and cell lines; Adherent and Suspension cells; subculturing of mammalian cells; cell culture media; safety

Proteins in Medicine and Disease:

The role played by proteins in medicine. Therapeutic proteins; Types of protein - related diseases, e.g. Prion proteins; cystic fibrosis; Methods of prevention and protection.

Protein Assays:

Types of assays; Sensitivity; Choosing an assay; Miniaturisation

Protein Purification:

Physical, chemical and functional features which may be exploited in purification (e.g. post translational modification); Sample preparation;Extraction from cells; Solubilisation of membrane proteins; Immuno-precipitation; Protein concentration - Salting out; TCA precipitation; Ultrafiltration;


Quantitation; Characterisation (Size / Subunits / Sequence); Factors affecting migration; Continuous and Discontinuous buffer systems; IEF and SDS electrophoresis; MW Determinations; Western Blotting.


Theory, principles and applications approaches to protein purification – Size-exclusion / Ion-exchange / gel filtration / Affinity / HPLC chromatography

Module Content & Assessment
Assessment Breakdown %
Formal Examination70
Other Assessment(s)30