Introduction to fundamental principles of biotechnology and recombinant DNA techniques, including key historical developments, commonly used techniques and applications in the pharmaceutical and nutraceutical sectors. The module aims to equip the student with practical and cognitive skills to perform basic molecular biology techniques in the associated and aligned laboratories.
Mechanisms of gene regulation in Prokaryotes and Eukaryotes. The role of RNA synthesis and processing in modulating gene expression
Introduction to recombinant DNA technology and molecular cloning methods, Golden Gate and Gibson assembly, CRISPR. Plasmid cloning vectors and their role in the formation of recombinant DNA. Transformation of recombinant vectors into expression systems. Selection/screening methods for recombinant DNA
Polymerase Chain Reaction (PCR) methodology and relevant applications
Properties of Expression systems used in industry
Industrial applications of gene cloning e.g. Monoclonal antibody production
Bioprocessing overview
Laboratory practicals: Aligned relevant practical based experimental protocols to reinforce the theory delivered in lectures
Lectures, Brightspace, Online resources
Pre-laboratory preparatory videos e.g. YouTube and quizzes outlining the learning outcomes.
For laboratory based practical sessions a large emphasis will be placed on planning, problem solving, trouble shooting in a group environment, technique development and scientific communication
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 40 |
| Other Assessment(s) | 60 |