Facilities
School of Food Science & Environmental Health
- Fermentation bioreactor (Solaris Jupiter 2.0 Pressure Control Vessel)
- BioFlo 415 Benchtop SIP Fermentor (New Brunswick Scientific)
- MMS Membrane Triple System (ThermaFlo)
- MiniJet Pro Piston Injection Molding System (Thermo Scientific™ HAAKE™)
- Thermogravimetric analysis with infrared and mass spectrometry (Perkin Elmer Pyris 1 Thermogravimetric analyser hyphenated to Frontier FT-IR and Clarus GC-MS; MiCRA)
- Shelf-life laboratory equipped with x4 fridge, x2 temperature and relative humidity
- controlled incubators and x2 freezer (SHRH)
- Microbiology laboratories; autoclaves, anaerobic/aerobic incubators, spiral
plater (EDIJET2), class II Type laminar flow cabinets x4, UV cabinet (SHRH)
- Gel preparation and electrophoretic equipment, PCR, RTPCR (7500FAST
- applied biosystems), thermocyclers, flow cytometer (CytoFLEX Beckmann Coulter) (SHRH)
- Multiplate reader fluorescence, luminescence and absorbance (Varioscan Lux) (SHRH)
School of Biological, Health and Sports Sciences
https://www.tudublin.ie/explore/faculties-and-schools/sciences-health/biological-health-and-sports-sciences/
School of Chemical & BioPharmaceutical Sciences
https://www.tudublin.ie/explore/faculties-and-schools/sciences-health/school-chemical-biopharmaceutical-sciences/
Pilot Plant:
The Fermentation suite can be set up for microbial cell culture and also has microspargers and marine-type impellers for conversion to animal cell culture. The suite has a 10L capacity, with steam-in-place (SIP) and clean-in-place (CIP) facilities, a 10L system, and a 2L quad fermentation unit.
All the fermenters have pH, P02, turbidity and off gas analysis which feeds back to a MFCS data handling unit. For downstream processing there is a centrifuge (50L/hr, 11,750 rpm) and a Millipore TFF unit for concentrating down product, depending on the process.
BIOSTAT Cultibag RM 20/50 is a wave technology/disposable bag system for animal cell culture (up to 20L capacity currently and up to 50L with additional equipment).
Solid formulations equipment includes 5 vessels ranging from 250-500L; training instrumentation skid, centrally controlled by a PLC with SCADA interface for the user.
Other equipment includes:
- Milipore K-Prime Unit (larger scale chromatography projects)
- Bottling line with 500ml fill capacity fed from the main vessels in the lab
- Pasteurising unit as part of the main lab with a 500L holding tank to maintain temperature
Sustainability & Health Research Hub:
- LC-qTOF MS (Agilent 1290 Infinity II hyphenated to Agilent 6545 Q-TOF; SHRH)
- LC-QqQ MS (Agilent 1290 Infinity II hyphenated to Agilent 6470 Triple Quad; SHRH)
- GC-MS/MS (Agilent 7890B GC hyphenated to Agilent 7000C Triple Quad, SHRH)
Additionally, PIs have access to the Irish Centre for High End Computing (ICHEC) facilities for bioinformatics and computational modelling work.