BD Accuri C6 Flow Cytometer
Overview:
Flow cytometry is an analytical technique used to measure the physical and fluorescent properties of individual cells or particles as they pass through a laser beam. It enables the rapid analysis of cell populations based on parameters such as cell size, internal complexity, and fluorescence.
The BD Accuri C6 Flow Cytometer is a benchtop, two-laser, six detector system designed for multiparametric cell analysis. It supports applications such as cell viability assessment, immunophenotyping, cell cycle analysis, and the detection of fluorescently labelled cellular markers.
The instrument supports a range of research applications, including cell viability and cytotoxicity assays, immunophenotyping, cell cycle analysis, and the assessment of fluorescently labelled cellular markers. It can be used to investigate cellular responses to drug treatments and other experimental conditions.
Technical Specifications:
| Laser Excitation | 488 nm & 640 nm |
| Laser Profile | 10 x 75 μm |
| Laser Power |
20 mW 488 nm Solid State Blue Laser 14.7 mW 640 nm Diode Red Laser |
| Scatter Detection |
Forward (0 degrees, +/- 13) Side (90 degrees +/- 13) |
| Emission Detection |
4 colors, standard optical filters
|
| Optical Alignment | Fixed Alignment |
| Flow Cell | 200 μm ID quartz capillary |
| Minimum Detectable Particle Size | 0.5 μm |
| Minimum Sample Volume | 300 μL (in 12 x 75 mm tube) |
| Custom Sample Flow Rates | 10 - 100 μL/min |
| Custom Core Diameter | 5 - 40 μm |
| Operating Ranges | 15 - 30 °C; < 80% relatively humidity |
Fluorescence Sensitivity:
| Fluorescence Sensitivity, (MESF) | FITC < 150; PE < 100 |
| Fluorescence Linearity | 2 +/- 0.05% for Chick Erythrocyte Nuclei (CEN) |
| Fluorescence Precision | <3% CV for CEN |
| Scatter Resolution | Resolves human peripheral blood lymphocytes, monocytes, and granulocytes. |
View the full instrument manual here.