ClarioStar Plus Microplate Reader

Overview:

A multimode microplate reader is used to measure and quantify optical signals from samples contained in microplates. Depending on the detection method, it can measure absorbance, fluorescence or luminescence, allowing researchers to analyse a wide range of biological, chemical and biochemical reactions.

The ClarioStar Plus is a multimodal microplate reader with advanced LVF Monochromators, highly sensitive filters, and an ultra-fast UV/Vis spectrometer. The Enhanced Dynamic Range and automatic focus technologies make manual settings superfluous and detection optimisation easier than ever. Automatic Control Unit, temperature incubation, multiple shaking, well-scan and reagent injectors make the ClarioStar Plus the ideal platform for any live cell-based assay.

Modes of measurement options available on the ClarioStar Plus plate reader:

Fluorescence Intensity
UV/Vis Absorbance
Luminescence
Time-Resolved Fluorescence (TRF)
Fluorescence Polarisation (FP) in both Visible and UV range
Fluorescence Resonance Energy Transfer (FRET)
Time-Resolved FRET (TR-FRET)

The instrument also has additional capability of UV/Vis or Fluorescence application on sample volumes as low of 2 μL, commonly used for quantifying DNA, RNA and protein concentrations.

The instrument supports applications including cell viability and cytotoxicity assays, cell proliferation, enzyme activity, protein and nucleic acid quantification, fluorescence-based assays, and biochemical reaction monitoring.

Technical Specifications:

Measurement Modes	Top and Bottom reading Endpoint and Kinetic measurements Spectral scanning (fluorescence, luminescence and absorbance) Sequential multi excitation Sequential multi emission Ratiometric measurements Well Scanning
Microplate Format	6- to 1536- well plates LVis Plate with 16 low volume microspots (2 μL) Cuvette port x 2
Light Source	High-energy Xenon flash lamp Dedicated laser for AlphaScreen/ AlphaLISA/ AlphaPlex
Detectors	Low noise photomultiplier tube (PMT) Red-sensitive photomultiplier tube UV/Vis absorbance spectrometer
Monochromator	Luminescence Top and Bottom Fluorescence excitation/ Emission spectral scanning Luminescence emission scanning Spectral range: 320 - 840 nm Software selectable bandwidth: 8 - 100 nm
Linear Variable Dichroic Mirror	Spectral range: 340 - 760 nm
UV/Vis Absorbance Spectrometer	Spectral scanning or upto 8 discrete wavelenghts Spectral range: 220 - 1000 nm Bandwidth: 3 nm Accuracy: <1% at 2OD Precision: <0.5% at 1OD and <0.8% at 2OD. Linearity: <0.8% at 2OD
Fluorescence Intensity (FI)	With filters: Top read <0.15 pM FITC and Bottom read <1.0 pM. With monochromater: Top read <0.35 pM (<7 amol/well FITC) and Bottom read < 3.0 pM (<150 amol/well FITC)
TRF	<20 fM europium, 384, 80 μL (<1.6 amol/well)
Fluorescence Polarisation (FP)	<0.5 mP SD at 1 nM FITC (384sv black 20 μL) and <1.5 mP SD at 1 nM FITC (1536 black 8 μL)
Luminescence	< 0.4 pM (< 8 amol/well ATP 384 sv white, 20ul)
Temperature Control	+3 to 45 °C
Shaker Options	Orbital Linear Double orbital Programmable shaker speed between 50 to 700 rpm.