Structural and mechanistic insights into activators of alcohol dehydrogenase
This review unifies all known ADH activation mechanisms, linking physiology and biocatalysis while highlighting structural ambiguities, translational limits, and future research directions.
This review presents the first comprehensive synthesis of all known modes of alcohol dehydrogenase (ADH) activation, bridging physiological mechanisms with biocatalytic applications. It critically examines the challenges in pinpointing peptide activator binding sites, underscoring the structural ambiguities that complicate mechanistic interpretation. The review also addresses the limitations inherent in extrapolating in vitro findings to in vivo contexts, emphasising the need for more integrative models. Concluding with forward-looking perspectives, the review outlines promising directions for future research, including advanced structural mapping and translational studies to enhance ADH-based biocatalysis.